Fibronectin (mouse) ELISA Kit

Fibronectin is a 430 KDa dimeric glycoprotein that exists in 2 forms - cellular and plasma fibronectin. BioVision’s mouse Fibronectin ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse Fibronectin is coated on a 96-well plate. Standards and test samples are added to the wells and Fibronectin present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for Fibronectin is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Fibronectin captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 15 pg/ml.

• Detection method: Absorbance (450 nm) • Species reactivity:mouse • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay for mouse Fibronectin. • Detection Range:156 pg/ml – 10,000 pg/ml. Sensitivity: < 15 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.

Fibronectin ELISA kit

Fibronectin (mouse) ELISA Kit

• 96 wells coated with anti-mouse monoclonal Fibronectin antibody, 1 Microplate • Lyophilized native mouse Fibronectin standard (10 ng/vial) • Biotinylated anti-mouse Fibronectin antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution

-20°C

gel pack

12 months

mouse

Application- Quantitative protein detection, establishing normal range

• Serum & plasma (heparin, EDTA, Citrate) • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus