IL-6 (rat) ELISA Kit

Catalog number
K4145-100
Name
IL-6 (rat) ELISA Kit
Size
100 assays
Price
834.00 EUR
Supplier

Details

Kit's description
BioVision’s rat IL-6 ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody specific for rat IL-6 coated on a 96-well plate. Standards (E. coli F25-T211) and test samples are added to the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for IL-6 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse IL-6 captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 5 pg/ml.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: rat • Application: Quantitative protein detection, establishing normal range etc.
Kit's benefits
• Easy, convenient and time-saving method to assay for rat IL-6. • Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 5 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.
Category
IL-6
Kit's other name
none
Contents
• IL-6 Microplate coated with anti-rat monoclonal Ab against IL-6, 96 wells • Lyophilized recombinant rat IL-6 standard (10 ng/vial) • Biotinylated anti-rat IL-6 antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
rat
Applications
Application- Quantitative protein detection, establishing normal range.
Samples tested
• Serum & plasma (heparin, EDTA, citrate) • Cell culture supernatants
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Latin name
Rattus norvegicus