PD-1 (human) ELISA Kit

Human PD-1 (Programmed Death-1) ELISA kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of human PD-1 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human PD-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and PD-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PD-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PD-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of PD:1 in huam serum, plasma, and cell culture supernatants.

Easy, convenient and time-saving method to measure the level of PD-1 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.156 - 10 ng/ml Sensitivity: < 0.094 ng/ml

ELISA kit

PD-1 (human) ELISA Kit

• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Human

Quantitative measurement of PD-1

Serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED