CTLA4 /CD152 (Human) ELISA Kit

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-HumanCTLA4 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CTLA4 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CTLA4 amount of sample captured in plate.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of CTLA4 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Easy, convenient and time-saving method to measure the level of CTLA4 inserum, plasma, tissue homogenates, culture supernatants and other biological fluids.

ELISA kit

CTLA4 /CD152 (Human) ELISA Kit

• Microplate: 96 wells, coated with anti-human CTLA4 • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Human

Quantitative measurement of CTLA4 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Human serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED