3-Nitrotyrosine ELISA Kit

3-Nitrotyrosine (3-NT) is a biomarker of nitrogen free radical species modified proteins in systemic autoimmunogenic conditions. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with 3-NT. During the reaction 3-NT in the sample or standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 3-NT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Detection method: Absorbance (450 nm) • Species reactivity: Universal • Application: quantitative measurement of 3:Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Easy, convenient and time-saving method to measure the level of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

ELISA kit

3-Nitrotyrosine ELISA Kit

• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Universal

Quantitative measurement of 3-Nitrotyrosine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Serum, plasma, tissue homogenates, cell culture supernatant and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED