Histamine (HIS) ELISA Kit

Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine is involved in the inflammatory response and has a central role as a mediator of pruritus. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues. This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with histamine. During the reaction, histamine in the sample or standard competes with a fixed amount of histamine on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to histamine. The concentration of histamine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of histamine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Easy, convenient and time-saving method to measure the level of histamine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 1.563 - 100 ng/ml Sensitivity: < 0.938 ng/ml

ELISA kit

Histamine ELISA Kit

• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Universal

Quantitative measurement of histamine in serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

Serum, plasma, tissue homogenates, culture supernatants and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

histidine