Phospho-Akt (Ser473) ELISA Kit

Phospho-Akt (Ser473) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated Akt protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-Akt (Ser473). An anti-pan Akt antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Akt present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Akt (Ser473) antibody is used to detect phosphorylated Akt (Ser473). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Akt (Ser473) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of Phospo:Akt (Ser473) in human cell lysates

• Easy, convenient and time-saving method to measure the level of Phospho- Akt (Ser473)

ELISA kit

Phospho-Akt ELISA Kit

• Akt Microplate (Item A) coated with anti-pan Akt • Wash Buffer Concentrate (20x) (Item B) • Assay Diluent (5X) (Item E2) • Detection Antibody Akt (Ser473) (Item C) • HRP-conjugated Anti-rabbit IgG (500X) (Item D-1) • TMB One-Step Substrate Reagent (Item H) • Stop Solution (Item I) • Cell Lysate Buffer (2X) (Item J) • Positive Control A431 (Item K): lyophilized powder from A431 cell lysate

-20°C

gel pack

12 months

Human

Quantitative measurement of Phospo-Akt (Ser473) in human cell lysates

Cell Lysates

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED