PARP1 (Human) ELISA Kit

Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. BioVision’s PARP1 ELISA kit is a sandwich ELISA assay for the quantitative measurement of human PARP1 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of PARP1 captured from the samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of PARP:1 in human serum, plasma, tissue homogenates and other biological fluids.

Easy, convenient and time-saving method to measure the level of PARP-1 in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.625 – 40 ng/ml Sensitivity: < 0.375 ng/ml

ELISA kit

none

• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Human

Quantitative measurement of PARP-1 in human serum, plasma, tissue homogenates and other biological fluids.

serum, plasma, tissue homogenates and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED