SMAD2 (Human) ELISA Kit

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. BioVision’s SMAD2 ELISA kit is a sandwich ELISA assay for the quantitative measurement of human SMAD2 in serum, plasma and cell culture supernatants. The density of color is proportional to the amount of SMAD2 captured from the samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of SMAD2 in human serum, plasma, tissue homogenates and other biological fluids.

Easy, convenient and time-saving method to measure the level of SMAD2 in human serum, plasma, tissue homogenates, culture supernatants and other biological fluids. Detection Range: 0.312-20 ng/ml Sensitivity: < 0.188 ng/ml

ELISA kit

none

• Microplate: 96 wells • Lyophilized Standard • Sample / Standard dilution buffer • Biotin- detection antibody (Concentrated) • Antibody dilution buffer • HRP-Streptavidin Conjugate (SABC) • SABC dilution buffer • TMB substrate • Stop Solution • Wash buffer (25X) • Plate sealers

4°C

gel pack

6 months

Human

Quantitative measurement of SMAD2 in human serum, plasma, tissue homogenates and other biological fluids.

Human serum, plasma, tissue homogenates and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED