Phospho-EGFR (Tyr 1069) ELISA Kit

Phospho-EGFR (Tyr1069) ELISA (Enzyme-Linked Immunosorbent Assay) kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated EGFR protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-EGFR (Tyr1069). An anti-EGFR antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated EGFR present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-phospho-EGFR (Tyr1069) antibodies are used to detect phosphorylated EGFR. After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG (secondary antibody) is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EGFR bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of phosoho:EGFR (Tyr1069) in human cell lysates

Easy, convenient and time-saving method to measure the level of phosoho-EGFR (Tyr1069) in human cell lysates

ELISA kit

none

• EGFR Microplate (Item A) coated with monoclonal anti-EGFR • Wash Buffer Concentrate (20X) (Item B) • Assay Diluent (5X) (Item E2) • Anti-phospho-EGFR (Tyr1069) (Item C) • HRP-conjugated Anti-rabbit IgG (1000X) (Item D-1) • TMB One-Step Substrate Reagent (Item H) • Stop Solution (Item I) • Cell Lysate Buffer (2X) (Item J) • Positive Control A431S001-1 (Item K)

-20°C

gel pack

6 months

Human

Quantitative measurement of phosoho-EGFR (Tyr1069) in human cell lysates

Human cell lysate

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED