Phospho-SMAD5 (Ser463/465) ELISA Kit

Phospho-SMAD5 (Ser463/465) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated SMAD5 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-SMAD5. An anti-pan SMAD5 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and SMAD5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-SMAD5 (Ser463/465) antibody is used to detect phosphorylated SMAD5. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SMAD5 (Ser463/465) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of phosoho:SMAD5 (Ser463/465) in human cell lysates

Easy, convenient and time-saving method to measure the level of phosoho-SMAD5 (Ser463/465) in human cell lysates

ELISA kit

none

Microplate (Item A) coated with anti-pan SMAD5 antibody Wash Buffer Concentrate (20X) (Item B) Assay Diluent (5X) (Item E2) Detection Antibody SMAD5 (S463/465) (Item C-1) HRP-conjugated Anti-rabbit IgG (1000X) (Item D-1) TMB One-Step Substrate Reagent (Item H) Stop Solution (Item I) Cell Lysate Buffer (2X) (Item J) Positive Control (Item K): lyophilized powder from Hela cell lysate

-20°C

gel pack

6 months

Human

Quantitative measurement of phosoho-SMAD5 (Ser463/465) in human cell lysates

Human cell lysate

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED