TGF-beta1 (rat) ELISA Kit

TGF-β1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. BioVision’s rat TGF-β1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for rat TGF-β1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-β1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-β1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the rat TGF-β1 captured onto the plate. This ELISA kit shows

• Detection method: Absorbance (450 nm) • Species reactivity:rat • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and reliable method to assay for rat TGF-beta 1. • Detection Range: 15.6-1000 pg/ml. Sensitivity: < 1 pg/ml. • This ELISA kit shows

TGF-beta, TGF beta, Transforming growth factor beta

Transforming growth factor beta 1 (rat) ELISA Kit

• 96 wells coated with anti-rat TGF-β1 antibody, 1 Microplate • Lyophilized recombinant rat TGF-β1 (25KDa) standard (10 ng/vial) • Biotinylated anti-rat TGF-β1 antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution

-20°C

gel pack

12 months

rat

Application- Quantitative protein detection, establishing normal range.

• Serum & plasma (EDTA) • Cell culture supernatants, urine

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus