CXCL12/SDF-1 alpha (human) ELISA Kit

BioVision’s Human SDF-1 (Stromal cell-derived factor-1) alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SDF-1 alpha. This assay employs an antibody specific for human SDF-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human SDF-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. Sensitivity: 80 pg/ml. Detection Range: 80 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates and additional reagents allow for use in multiple experiments.

• Easy, convenient and reliable method to measure human CXCL12/SDF-1 alpha. • Detection Range: 80 pg/ml – 6,000 pg/ml.

CXCL12 ELISA kit, SDF-1 alpha ELISA kit

Stromal cell-derived factor-1 ELISA Kit, C-X-C motif chemokine 12 ELISA Kit

• Microplate coated with anti-human SDF-1 alpha • Wash Buffer (20x) (Item B) • Human SDF-1 alpha Standard (Item C) • Assay Diluent A (Item D) (0.09% NaN3) • Assay Diluent B (Item E) (5x concentrated) • Detection Antibody SDF-1 alpha (Item F) • HRP-Streptavidin (Item G), (200x) • TMB (Item H) • Stop Solution (Item I)

-20°C

gel pack

12 months

human

Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates and additional reagents allow for use in multiple experiments.

• Plasma (human SDF-1 alpha conc. is pretty low in normal plasma, it may not be detected. serum samples are not recommended) • Cell culture supernatants • Urine

The CXCL12/SDF-1 alpha ( ) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED