QuickDetect™ CD40 Ligand (Human) ELISA Kit

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Induces tyrosine phosphorylation of isoform 3 of CD28. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching. BioVision’s QuickDetect™ CD40 Ligand ELISA kit is a sandwich ELISA assay for measuring the quantity of human CD40 Ligand in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

• Detection method: Absorbance (450 nm)• Species reactivity: Human• Application: quantitative measurement of CD40L in human serum, plasma, cell culture supernatant, and other biological fluid.

Easy, convenient and time-saving method to measure the level of CD40L in human serum, plasma, culture supernatants and other biological fluids.The entire assay can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.Detection Range: 18.7 - 600 ng/ml

ELISA kit

none

• Micro ELISA Plate• Standard• Standard Diluent• Special Diluent• HRP-Conjugate Reagent• Chromogen Solution A• Chromogen Solution B• Stop Solution • Wash buffer (20X)• Sealed bags

4°C

gel pack

6 months

Human

Quantitative measurement of CD40L in human serum, plasma, cell culture supernatant and other biological fluids.

Human serum, plasma, tissue homogenates and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.