QuickDetect™ 24(S)-Hydroxycholesterol (Human) ELISA Kit

An important cholesterol metabolite (cholesterol 24-hydroxylase) found in the brain. It is an endogenous agonist of the nuclear receptor LXR. It has been shown to induce cell death in neuroblastoma cells, possibly via a necroptosis pathway. 24(S)-Hydroxycholesterol has also been found to be a high affinity ligand for RORα and RORγ (Ki = 25 nM). 24(S)-Hydroxycholesterol levels are elevated in the cerebral spinal fluids of patients with neurogenerative diseases suggesting possible clinical applications. It was also able to reduce Aβ production and increase ER-resident immature amyloid precursor protein (APP) levels in human neuroblastoma SH-Sy5Y cells and CHO cells via inhibition of intracellular amyloid precursor protein trafficking. BioVision’s QuickDetect™ 24-OHC ELISA kit is a sandwich ELISA assay for measuring the quantity of human 24-OHC in serum, plasma and cell culture and other biological fluid. The entire process can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results.

• Detection method: Absorbance (450 nm) • Species reactivity: Human • Application: quantitative measurement of 24:OHC in human serum, plasma, cell culture supernatant, and other biological fluid.

Easy, convenient and time-saving method to measure the level of 24-OHC in human serum, plasma, culture supernatants and other biological fluids. The entire assay can be performed within 90 minutes with only a single incubation and a wash step respectively, resulting in fewer handling steps, fewer errors and more consistent results. Detection Range: 0.5 -100 ng/ml

ELISA kit

none

• Micro ELISA Plate • Standard • Standard Diluent • Special Diluent • HRP-Conjugate Reagent • Chromogen Solution A • Chromogen Solution B • Stop Solution • Wash buffer (20X) • Microplate Sealers • Sealed bags

4°C

gel pack

6 months

Human

Quantitative measurement of 24-OHC in human serum, plasma, cell culture supernatant and other biological fluids.

Human serum, plasma, cell culture supernatant, tissue homogenate and other biological fluids.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED