Neuregulin-1/NRG1-beta1 (human) ELISA Kit

BioVision’s human NRG-1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human NRG-1 is coated on a 96-well plate. Standards and test samples are added to the wells and NRG-1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal detection antibody from goat specific for NRG-1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, HRP conjugate is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is used by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human NRG-1 captured onto the plate. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 62.5 pg/ml – 4000 pg/ml. Sensitivity: < 10 pg/ml. Coefficient of variation: 5% (Intra-assay) and 7% (Inter-assay).

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative measurement of human Neuregulin:1/NRG:1

• Easy, convenient and time-saving method to assay for human Neuregulin-1. • Sensitivity:

Neuroscience, Neuregulin-1, NRG-1, NRG-1beta

Neuregulin-1 ELISA kit, Human Neuregulin-1 ELISA kit, Human Neuregulin1 ELISA kit, NRG-1 ELISA kit, NRG-1-beta-1 ELISA kit, NRG-1 beta ELISA kit

• NRG-1 Ab coated plate • Human NRG-1 Standard • Detection antibody (100x) • HRP Conjugate (100x) • Sample diluent buffer • Antibody diluent buffer • HRP diluent buffer • TMB substrate • Stop solution

-20°C

gel pack

12 months

human

Quantitative measurement of human Neuregulin-1/NRG-1

• Serum and plasma • Cell culture supernatant

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED