Progranulin (mouse) ELISA Kit

BioVision’s Progranulin (mouse) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Mouse

The lowest level of progranulin that can be detected by this assay is 60 pg/ml. The Assay range is 0.125 ng/ml – 8 ng/ml. This ELISA is specific for the measurement of natural and recombinant mouse progranulin. It does not cross-react with mouse RBP4, mouse adiponectin, mouse Nampt, mouse leptin, mouse RELM- β, mouse IL-33, mouse IDO, mouse resistin, mouse clusterin, mouse ANGPTL3, mouse ANGPTL4, human vaspin, human adiponectin, human progranulin, rat Nampt.

Progranulin Quantitation

none

• 1 plate coated with mouse progranulin Antibody (12 x 8-well strips) • 1 bottle Wash Buffer 10X (50 ml) • 1 bottle Diluent 5X (50 ml) • 1 bottle Detection Antibody (12 ml) • 1 vial Detector 100X (HRP Labeled Streptavidin) (150 μl) • 1 vial human progranulin Standard (lyophilized) (8 ng) • 1 vial human progranulin QC sample (lyophilized) • 1 bottle TMB Substrate Solution (12 ml) • 1 bottle Stop Solution (12 ml) • 3 plate sealers (plastic film)

-20°C

gel pack

12 months

see datasheet

Inquire

Serum and Cell culture supernatants.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus