Progranulin (rat) ELISA Kit

BioVision’s Progranulin (rat) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Rat

The lowest level of progranulin that can be detected by this assay is 40 pg/ml. The Assay range is 0.063 ng/ml – 4 ng/ml. This ELISA is specific for the measurement of natural and recombinant rat progranulin. It does not cross-react with human progranulin, human granulin C, human granulin F, rat RBP4, rat adiponectin, rat Nampt, rat ANGPTL4, rat lipocalin-2, rat resistin, mouse RBP4, mouse adiponectin, human RBP4, human adiponectin.

Progranulin Quantitation

none

• 1 plate coated with rat progranulin Antibody (6 x 16-well strips) • 1 bottle Wash Buffer 10X (2x30 ml) • 1 bottle Diluent 10X (2x30 ml) • Detection Antibody (20 μl ) • 1 vial Detector (HRP Labeled Streptavidin) (lyophilized) • 1 vial rat progranulin Standard (lyophilized) (8 ng) • 1 bottle TMB Substrate Solution (12 ml) • 1 bottle Stop Solution (12 ml) • 2 plate sealers (plastic film)

4°C

gel pack

12 months

see datasheet

Inquire

Serum and Cell culture supernatants.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus