Progranulin (human) ELISA Kit

BioVision’s Progranulin (human) ELISA kit is a sandwich Enzyme Linked-Immunosorbent Assay (ELISA) for quantitative determination of human progranulin in biological fluids. A polyclonal antibody specific for progranulin has been precoated onto the 96-well microtiter plate. Standards and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, progranulin is recognized by the addition of a biotinylated polyclonal antibody specific for progranulin (Detection Antibody). After removal of excess biotinylated antibody, HRP labeled streptavidin (Detector) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’-tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of progranulin in the samples.

• Detection method: Absorbance (450 nm) • Species reactivity: Human

The lowest level of progranulin that can be detected by this assay is 32 pg/ml. The Assay range is 0.063 ng/ml – 4 ng/ml. This ELISA is specific for the measurement of natural and recombinant human progranulin. It does not cross-react with human RBP4, human adiponectin, human Nampt, human leptin, human RELM-β, human ANGPTL6, human FABP4, human TNF-α, human IL-33, human GPX3, human resistin, human clusterin, human vaspin, human PAl1, human ANGPTL3, mouse Nampt, rat Nampt.

Progranulin Quantitation

none

• 1 plate coated with human progranulin Antibody (12 x 8-well strips) • 1 bottle Wash Buffer 10X (50 ml) • 1 bottle Diluent 5X (50 ml) • 1 bottle Detection Antibody (12 ml) • 1 vial Detector 100X (HRP Labeled Streptavidin) (150 μl) • 1 vial human progranulin Standard (lyophilized) (8 ng) • 1 vial human progranulin QC sample (lyophilized) • 1 bottle TMB Substrate Solution (12 ml) • 1 bottle Stop Solution (12 ml) • 3 plate sealers (plastic film)

-20°C

gel pack

12 months

see datasheet

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Serum, Plasma, Urine, Cell culture supernatants and Cerebrospinal Fluid (CSF).

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED