IL-15 (human) ELISA Kit

IL-15 is a recently identified novel cytokine that shares many biological properties with IL-2, including T-Cell, B-Cell and NKcell-stimulatory activities. BioVision’s Human IL-15 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-15. This assay employs an antibody specific for human IL-15 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-15 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-15 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-15 is typically less than 3 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: Human

• Easy, convenient and time-saving method to assay for human IL-15. • The minimum detectable dose of IL-15 is typically less than 3 pg/ml. • This ELISA kit is specific for the measurement of human IL-15. The kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1alpha, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1alpha, MIP-1 β, MIP-1, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-alpha, TNF-β, TPO, VEGF).

IL-15

none

• IL-15 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human IL-15. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials of recombinant human IL-15. • Assay Diluent A (Item D): 30 ml, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. • Detection Antibody IL-15 (Item F): 2 vial of biotinylated anti-human IL-15 (each vial is enough to assay half microplate). • HRP-Streptavidin concentrate (Item G): 200 μl of 200x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’- tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.

-20°C

gel pack

12 months

see datasheet

Inquire

• Serum, plasma (collect plasma using EDTA or heparin as an anticoagulant. Citrate plasma is not recommended for this assay) • Cell culture supernatants • Urine

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED