MMP-8 (rat) ELISA Kit

BioVision’s Rat MMP-8 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat MMP-8. This assay employs an antibody specific for MMP-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat MMP-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of MMP-8 is typically less than 50 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: Rat

• Easy, convenient and time-saving method to assay for rat MMP-8. • The minimum detectable dose of MMP-8 is typically less than 50 pg/ml. • This ELISA kit is specific for the measurement of rat MMP-8. The kit shows no cross-reactivity with rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α.

MMP-8

Neutrophil Collagenase (rat) ELISA Kit, PMNL Collagenase (rat) ELISA Kit

• MMP-8 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-rat MMP-8. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials, recombinant rat MMP-8. • Assay Diluent A (Item D): 30 ml of diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent. • Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. • Detection Antibody MMP-8 (Item F): 2 vial of biotinylated anti-MMP-8 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate (Item G): 200 µl of 160x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid.

-20°C

gel pack

12 months

see datasheet

Inquire

• Serum & plasma (using heparin as an anticoagulant, EDTA & Citrate are not recommended) • Cell culture supernatants • Urine

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus