BMP-4 (human) ELISA Kit (For Lysates)

BioVision’s Human BMP-4 (Bone morphogenetic proteins-4) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BMP-4. This assay employs an antibody specific for human BMP-4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BMP-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: Human

• Easy, convenient and time-saving method to assay for human BMP-4 in cells & tissues. • The minimum detectable dose of BMP-4 is typically less than 15 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, BMP-6, BMP-7, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.

BMP-4

Bone morphogenetic protein 4 (human) ELISA Kit

• BMP-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human BMP-4. • Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution. • Standards (Item C): 2 vials, recombinant human BMP-4. • Sample Diluent Buffer (Item D): 10 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissue lysate) diluent. • Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Detection Antibody (Item F) & HRP-Streptavidin Concentrate (Item G) diluent. • Detection Antibody BMP-4 (Item F): 2 vial of biotinylated anti human BMP-4 (each vial is enough to assay half microplate). • HRP-Streptavidin Concentrate (Item G): 200 µl of 80x concentrated HRP-conjugated streptavidin. • TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’tetramethylbenzidine (TMB) in buffered solution. • Stop Solution (Item I): 8 ml of 0.2 M sulfuric acid. • Cell lysate buffer (Item J): 5 ml 2X cell lysate buffer.

-20°C

gel pack

12 months

see datasheet

Inquire

• Cell lysate & tissue lysate

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED