Nesfatin (human/mouse/rat) EIA Kit

BioVision’s Nesfatin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Nesfatin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Nesfatin antibody, both biotinylated Nesfatin peptide and peptide standard or targeted peptide in samples interacts competitively with the Nesfatin antibody. Uncompeted (bound) biotinylated Nesfatin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Nesfatin peptide in the standard or samples. This is due to the competitive binding to Nesfatin antibody between biotinylated Nesfatin peptide and peptides in standard or samples. A standard curve of known concentration of Nesfatin peptide can be established and the concentration of Nesfatin peptide in the samples can be calculated accordingly. EIA-NES-1 detects 420aa Nesfatin protein. No other active isoforms have been reported. The minimum detectable concentration of Nesfatin is 147 pg/ml or 3.09 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: Human, Mouse • Application: Quantitative protein detection, establishing normal range, validation of antibody array results.

Nesfatin

none

-20°C

gel pack

6 months

Species reactivity- human, mouse, rat

Quantitative protein detection, establishing normal range, validation of antibody array results.

• Serum & plasma • Cell culture media etc.

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus,Rattus norvegicus

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.