Periostin/OSF-2 (human) ELISA Kit

BioVision’s Periostin (human) ELISA kit is to be used for the in vitro quantitative determination of human periostin in cell culture supernatants, serum and plasma. A monoclonal antibody specific for periostin has been precoated onto the 96-well microtiter plate. Standards (STD) and samples are pipetted into the wells for binding to the coated antibody. After extensive washing to remove unbound compounds, periostin is recognized by the addition of a biotinylated monoclonal antibody specific for periostin (DET). After removal of excess biotinylated antibody, streptavidine-peroxidase (STREP-HRP) is added. Following a final washing, peroxidase activity is quantified using the substrate 3,3’,5,5’- tetramethylbenzidine (TMB). The intensity of the color reaction is measured at 450 nm after acidification and is directly proportional to the concentration of periostin in the samples. Detection limit: 15 pg/ml. Note: The Limit of detection was measured by adding three standard deviations to the mean value of 50 zero standard. Assay Range: 78 pg/ml – 5000 pg/ml. Linearity: Different samples containing human periostin were diluted several fold (1/50 to 1/100) and the measured recoveries ranged from 95% to 105%. Human periostin levels range in plasma or serum from 10 to >100 ng /ml.

• Detection method: Absorbance (450 nm) • Species reactivity: human. Cross react with mouse • Application: Quantitative ELISA

• Easy, convenient and time-saving method to assay Periostin. • The minimum detectable concentration of Irisin is 15 pg/ml. • This ELISA is specific for the measurement of natural and recombinant human periostin. It has been tested on human periostin isoforms 1 and 2 (should detect human isoforms 3 and 4). It does cross-react with mouse periostin.

Periostin

Osteoblast-specific Factor 2 (human) ELISA Kit, OSF-2 (human) ELISA Kit , Postn (human) ELISA Kit

• Periostin Ab-coated plate • Human Periostin Standard (1 μg) • Detection Antibody • HRP-Streptavidin (2 μg) • 10X Wash Buffer • 10X ELISA Buffer • TMB • Stop Solution • Plate Covers

4°C

gel pack

human, mouse

Quantitative ELISA

• Serum & plasma • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED