CRP (rat) ELISA Kit

BioVision’s Rat CRP (C Reactive Protein) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CRP. This assay employs an antibody specific for Rat CRP coated on a 96-well plate. Standards and samples are pipetted into the wells and CRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The minimum detectable dose of CRP is typically less than 0.2 ng/ml. Detection Range: 0.2 ng/ml – 60 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: rat • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay CRP. • The minimum detectable concentration of CRP is less than 0.2 ng/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α.

C Reactive Protein

C-Reactive Protein (rat) ELISA Kit

• CRP Microplate (Item A) coated with anti-Rat CRP, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant Rat CRP) • Assay Diluent (Item E) (5x concentrated) for Standard/Sample (serum/plasma/cell culture medium) diluent • Detection Antibody CRP (Item F), biotinylated anti-Rat CRP (one vial enough to assay half plate) • HRP-Streptavidin Concentrate (Item G), 500x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution • Stop Solution (Item I), 0.2 M sulfuric acid

-20°C

gel pack

12 months

rat

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma • Cell culture supernatants

C reactive proteins are detected by anti-CRP antibodies supplied by GENTAUR.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus