Resistin (human/mouse/rat) EIA Kit

BioVision’s Resistin [XCP-1 (CEBPE regulated myeloid-specific secreted cysteine-rich protein precursor 1), FIZZ3 (found in inflammatory zone 3), or ADSF (adipocyte-specific secretory factor)] Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Resistin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti-Resistin antibody, both biotinylated Resistin peptide and peptide standard or targeted peptide in samples interacts competitively with the Resistin antibody. Uncompeted (bound) biotinylated Resistin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Resistin peptide in the standard or samples. This is due to the competitive binding to Resistin antibody between biotinylated Resistin peptide and peptides in standard or samples. A standard curve of known concentration of Resistin peptide can be established and the concentration of Resistin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Resistin is 446 pg/ml. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: human, mouse, rat • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay Resistin. • The minimum detectable concentration of Resistin is less than 446 pg/ml. • This EIA kit detects Resistin (90 aa). No other active isoforms have been reported. This kit shows no cross-reactivity with the following cytokines tested: e.g., Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Resistin

XCP-1 (human/mouse/rat) EIA Kit, FIZZ3 (human/mouse/rat) EIA Kit , ADSF (human/mouse/rat) EIA Kit

• Resistin Microplate (Item A) coated with secondary antibody, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standard Resistin Peptide (Item C) (10 μl/vial) • Anti-Resistin polyclonal antibody (item N) (5 μl/vial) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium or other sample) diluent. (5x concentrated) • Biotinylated Resistin peptide (Item F), (20 μl/vial) • HRP-Streptavidin Concentrate (Item G), 160x concentrated • Positive Control (Item M) (100 μl) • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid

-20°C

gel pack

6 months

human, mouse, rat

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma • Culture supernatants & cell lysates

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus,Rattus norvegicus

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.