DPP IV (Mouse) ELISA Kit

BioVision’s Mouse DPP IV ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse DPP IV. This assay employs an antibody specific for DPP IV coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP IV present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse DPP IV antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP IV bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of DPP IV is typically less than 0.45 ng/ml. Detection Range: 0.45 ng/ml - 100 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: Mouse • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay DPP 4. • The minimum detectable concentration of DPP 4 is less than 0.45 ng/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested:Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, RANTES, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TNF RII, TPO, VCAM-1, VEGF.

DPP 4

CD26 (Mouse) ELISA Kit, Dipeptidyl peptidase-4 (Mouse) ELISA Kit, adenosine deaminase complexing protein 2 (Mouse) ELISA Kit

• DPP IV Ab coated Microplate (Item A), 96 wells • Wash Concentrate (20x) (Item B) • Mouse DPP IV Standards (Item C) • Assay Diluent C (Item L) • Assay Diluent B (5x) (Item E) • Biotinylated anti-mouse DPP IV Ab (Item F) • HRP-Streptavidin (200x) (Item G) • TMB (Item H) • Stop Solution (Item I)

-20°C

gel pack

12 months

Mouse

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & Plasma • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus