TNF-alpha (human) ELISA Kit

Catalog number
K4779-100
Name
TNF-alpha (human) ELISA Kit
Size
100 assays
Price
909.00 EUR
Supplier

Details

Kit's description
BioVision’s Human TNF-alpha (Tumor necrosis factor-alpha) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-alpha. This assay employs an antibody specific for human TNF-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNF-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNF-alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ1, TGFβ2, TGFβ3, VEGF. The minimum detectable dose of TNF-alpha is typically less than 30 pg/ml. Detection Range: 30 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits
• Easy, convenient and time-saving method to assay TNF-alpha. • The minimum detectable concentration of TNF-alpha is less than 30 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested e.g., human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO- alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1alpha, IL-1beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1α, TIMP-1, TIMP-2, TNFβ, TGFβ1, TGFβ2, TGFβ3, VEGF.
Category
TNF-alpha
Kit's other name
Tumor Necrosis Factor alpha (human) ELISA Kit
Contents
• TNF-alpha Microplate (Item A) coated with anti-human TNF-alpha, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human TNF-alpha) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent (0.09% sodium azide as preservative) • Assay Diluent B (Item E) for Standard/Sample (cell culture medium/urine) diluent. (5x concentrated) • Detection Antibody TNF-alpha (Item F), biotinylated anti-human TNF-alpha (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 600x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
human
Applications
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples tested
• Serum & plasma (human TNF-alpha concentration is pretty low in normal serum & plasma, it may not be detected in this assay) • Cell culture supernatants • Urine
Description
The TNF-alpha ( ) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Gene
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.