MIP-1 alpha (human) ELISA Kit

BioVision’s Human MIP-1 alpha (Macrophage Inflammatory Protein-1 alpha) or chemokine ligand 3 (CCL3) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-1 alpha. This assay employs an antibody specific for human MIP-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIP-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, MCP-1, MCP-2, MCP-3, MDC, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of MIP-1 alpha is typically less than 6 pg/ml. Detection Range: 6 pg/ml – 6,000 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay MIP-1 Alpha. • The minimum detectable concentration of MIP-1 Alpha is less than 6 pg/ml. • This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF,BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, MCP-1, MCP-2, MCP-3, MDC, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.

MIP-1 Alpha

Macrophage Inflammatory Protein-1 alpha (human) ELISA Kit, Chemokine Ligand 3 (human) ELISA Kit, CCL3 (human) ELISA Kit

• MIP-1 alpha Microplate (Item A) coated with anti-human MIP-1 alpha, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human MIP-1 alpha) • Assay Diluent (Item E) for Standard/Sample (serum/plasma/cell culture medium/urine) diluent (5x concentrated) • Detection Antibody MIP-1 alpha (Item F), biotinylated anti-human MIP-1 alpha (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 400x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid

-20°C

gel pack

12 months

human

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma (recommended using EDTA or heparin plasma) • Cell culture supernatants • Urine

The MIP-1 alpha ( ) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED