MIP-1 beta (human) ELISA Kit

Catalog number
K4785-100
Name
MIP-1 beta (human) ELISA Kit
Size
100 assays
Price
909.00 EUR
Supplier

Details

Kit's description
BioVision’s Human MIP-1 beta (MIP-1 β) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MIP-1 β. This assay employs an antibody specific for human MIP-1 β coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-1 β present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MIP-1 β antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP-1 β bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α,TNF-β, TPO, VEGF). The minimum detectable dose of MIP-1 β is typically less than 2.5 pg/ml. Detection Range: 2.5 pg/ml – 1,000 pg/ml. The intra-Assay reproducibility is CV
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results
Kit's benefits
• Easy, convenient and time-saving method to assay MIP-1 beta. • The minimum detectable concentration of MIP-1 beta is less than 2.5 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF,GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α,TNF-β, TPO, VEGF).
Category
MIP-1 Beta
Kit's other name
Macrophage Inflammatory Protein-1 beta (human) ELISA Kit,
Contents
• MIP-1 β antibody-coated Microplate (Item A), 96 wells • Wash Buffer (20x) (Item B) • rh MIP-1 β Standards (Item C) • Assay Diluent A (Item D) • Assay Diluent B (5x) (Item E) • MIP-1 β Detection Antibody (Item F) • HRP-Streptavidin (600x) (Item G) • TMB (Item H) • Stop Solution (Item I)
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
human
Applications
Quantitative protein detection, establishing normal range, validation of antibody array results
Samples tested
• Serum & plasma • Cell culture supernatants • Urine
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED