Ghrelin (human, mouse, rat) EIA Kit

BioVision’s Ghrelin Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Ghrelin peptide based on the principle of Competitive Enzyme Immunoassay. The microplate in the kit is pre-coated with anti-rabbit secondary antibody. After a blocking step and incubation of the plate with anti- Ghrelin antibody, both biotinylated Ghrelin peptide and peptide standard or targeted peptide in samples interacts competitively with the Ghrelin antibody. Uncompeted (bound) biotinylated Ghrelin peptide then interacts with Streptavidin-horseradish peroxidase (SAHRP), which catalyzes a color development reaction. The intensity of colorimetric signal is directly proportional to the amount of biotinylated peptide-SA-HRP complex and inversely proportional to the amount of Ghrelin peptide in the standard or samples. This is due to the competitive binding of Ghrelin antibody between biotinylated Ghrelin peptide and peptides in standard or samples. A standard curve of known concentration of Ghrelin peptide can be established and the concentration of Ghrelin peptide in the samples can be calculated accordingly. The minimum detectable concentration of Ghrelin is 161 pg/ml or 12.46 pM. The detection range for the kit is 0.1-1,000 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: human, mouse, rat • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay Ghrelin. • The minimum detectable concentration of Ghrelin is less than 161 pg/ml . • This EIA kit shows no cross-reactivity with the following cytokines tested: e.g., Nesfatin, Angiotensin II, NPY and APC.

Ghrelin

none

• Ghrelin Microplate (Item A) coated with secondary antibody, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Lyophilized Standard Ghrelin Peptide (Item C) • Lyophilized anti-Ghrelin Polyclonal antibody (Item N) • 1 X Assay Diluent E (Item R) for Standard/Sample (Serum or plasma, cell culture medium etc.), 25 ml/vial • Lyophilized Biotinylated Ghrelin peptide (Item F) • HRP-Streptavidin Concentrate (Item G), 100x concentrated • Lyophilized Positive Control (Item M) • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid

-20°C

gel pack

6 months

human, mouse, rat

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma • Cell culture media or other sample types

This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.Ghrelin, GHRL gene, obestatin prepropeptide or lenomorelin from ghrelinergic cells in the gastrointestinal tract. GHRL is a neuropeptide in the central nervous system regulating appetite. Ghrelin is regulating the distribution and rate of use of body energy.

MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus,Rattus norvegicus

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.