IL-1α (human) ELISA Kit (for lysates)

BioVision’s Huma IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IL-1 alpha. This assay employs an antibody specific for human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The minimum detectable dose of IL-1 alpha is typically less than 0.5 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay IL-1 alpha. • The minimum detectable concentration of IL-1 alpha is less than 0.5 pg/ml . • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g., human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF).

IL-1 alpha

none

• IL-1 alpha Microplate (Item A) coated with anti-human IL-1 alpha, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant human IL-1 alpha) • Sample Diluent Buffer (Item D) for Standard/Sample (cell lysate/tissue lysate) diluent (5x concentrated) • Assay Diluent (Item E) for Detection Antibody & HRP-Streptavidin concentrate diluent (5x concentrated) • Detection Antibody IL-1 alpha (Item F), biotinylated anti-human IL-1 alpha (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 500x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid • Cell Lysate buffer (Item J) (2x concentrated)

-20°C

gel pack

12 months

human

Quantitative protein detection, establishing normal range, validation of antibody array results

• Cell lysates • Tissue lysates

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED