IL-1α (mouse) ELISA Kit

BioVision’s mouse IL-1 alpha ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IL-1 alpha. This assay employs an antibody specific for human IL-1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IL-1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF). The minimum detectable dose of IL-1 alpha is typically less than 10 pg/ml. Detection Range: 10 pg/ml – 3000 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity:mouse • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay IL-1 alpha. • The minimum detectable concentration of IL-1 alpha is less than 10 pg/ml. • This ELISA kit shows no cross-reactivity with any of the cytokines tested (e.g Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, IFN-γ, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 α, IL-1β, IL-13, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-5, M-CSF, MIG, MIP-1α, MIP-1γ, MIP-2, MIP-3β, MIP-3α, PF-4, P-Selectin, SCF, SDF-1α, TARC, TCA-3, TECK, TIMP-1, TNF RI, TPO, VEGF).

IL-1 alpha

none

• Mouse IL-1 alpha Ab coated plate (Item A), 96 wells • Wash Buffer Concentrate (20x) (Item B) • Mouse IL-1 alpha Standard (Item C) • Assay Diluent D (5x) (Item K) • Assay Diluent B (5x) (Item E) • Biotinylated anti-mouse IL-1 alpha Ab (Item F) • HRP-Streptavidin Concentrate (500x) (Item G) • TMB (Item H) • Stop Solution (Item I)

-20°C

gel pack

12 months

mouse

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus