IL-2 (rat) ELISA Kit

BioVision’s rat IL-2 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat IL-2. This assay employs an antibody specific for IL-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-rat IL-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α. The minimum detectable dose of IL-2 is typically less than 0.1 ng/ml. Detection Range: 0.1 ng/ml – 30 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity:rat • Application: Quantitative protein detection, establishing normal range, validation of antibody array results

• Easy, convenient and time-saving method to assay IL-2. • The minimum detectable concentration of IL-2 is less than 0.1 ng/ml. • This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1α, IL-1β, IL-4, IL-6, IL-10, GM-CSF, IFN-γ, Leptin, Lix, MCP-1, MIP-3α, β-NGF, TIMP-1, TNF-α.

IL-2

none

• IL-2 Microplate (Item A) coated with anti-rat IL-2, 96 wells • Wash Buffer Concentrate (20x) (Item B) • Standards (Item C) (recombinant IL-2) • Assay Diluent A (Item D) for Standard/Sample (serum/plasma) diluent. 0.09% sodium azide as preservative • Assay Diluent B (Item E) for Standard/Sample (cell culture medium) diluent. (5x concentrated) • Detection Antibody IL-2 (Item F), biotinylated anti-IL-2 (each vial enough for half plate) • HRP-Streptavidin Concentrate (Item G), 500x concentrated • TMB One-Step Substrate Reagent (Item H) 3,3’,5,5’-tetramethylbenzidine (TMB) in buffer solution • Stop Solution (Item I), 0.2 M sulfuric acid

-20°C

gel pack

12 months

rat

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma (rat IL-2 conc. is pretty low in normal serum/plasma, it may not be detected in this assay) • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus