VEGF (mouse) ELISA Kit

Vascular endothelial growth factor (VEGF) is a signaling messenger involved in endothelial cell proliferation and angiogenesis. BioVision’s mouse VEGF ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A rat monoclonal antibody specific for mouse VEGF is coated on a 96-well plate. Standards and test samples are added to the wells and VEGF present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for VEGF is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse VEGF captured onto the plate. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF. This ELISA kit shows no detectable cross-reactivity with related proteins. Detection Range: 15.6 pg/ml – 1000 pg/ml. Sensitivity: < 2 pg/ml.

• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Detection of IgG Ab to TPO

• Easy, convenient and time-saving method to assay for murine VEGF. • Detection Range: 15.6 pg/ml – 1000 pg/ml. • This ELISA kit shows no species cross-reactivity.

Vascular endothelial growth factor, VEGF

Vascular endothelial growth factor (mouse) ELISA kit, VEGF (mouse) ELISA kit

• Plate coated with anti-mouse VEGF antibody, 96-wells • Mouse VEGF standard (10 ng/vial), lyophilized • Biotinylated anti-mouse VEGF antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution

-20°C

gel pack

12 months

mouse

Quantitative protein detection, establishing normal range, validation of antibody array results

• Serum & plasma • Cell culture supernatants • Urine

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus