CCL2/MCP-1 (human) ELISA Kit

BioVision’s Human MCP-1 (Monocyte chemoattractant protein-1 also called Monocyte chemotactic protein-1) or chemokine ligand 2 (CCL2) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MCP-1. This assay employs an antibody specific for human MCP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MCP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human BDNF, BLC, ENA-78, FGF-4, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1α, MIP-1 β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF. The minimum detectable dose of MCP-1 is typically less than 2 pg/ml. Detection Range: 2 pg/ml – 500 pg/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity:human • Application: Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates and additional reagents allow for use in multiple experiments.

• Easy, convenient and time-saving method to assay for human CCL2/MCP-1 . • Detection Range: 2 pg/ml – 500 pg/ml

CCL2/MCP-1 ELISA kit

Monocyte chemoattractant protein-1 ELISA Kit, Monocyte chemotactic protein-1 ELISA Kit, chemokine ligand 2 ELISA Kit

• Plate coated with anti-human MCP-1 • Wash Buffer (20x) (Item B) • Human MCP-1Standard (Item C) • Assay Diluent (Item E) (5x) • Detection Ab MCP-1 (Item F) • HRP-Streptavidin (Item G) (400x) • TMB (Item H) • Stop Solution (Item I)

-20°C

gel pack

12 months

human

Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates and additional reagents allow for use in multiple experiments.

• Serum & plasma • Cell culture supernatants • Urine

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED