Cathepsin D (mouse) ELISA Kit

BioVision’s mouse Cathepsin D ELISA Kit is based on the standard sandwich enzyme-linked immunosorbent assay technology. This assay employs a monoclonal antibody form rat specific for Cathepsin D coated on a 96-well plate. Standards (NSO, I21-L410) and test samples are added to the wells and Cathepsin D present in a sample is bound to the wells by the immobilized antibody. A biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, Avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the mouse Cathepsin D captured onto the plate. This ELISA kit shows no cross-reactivity with other relevant proteins. Detection Range: 156 pg/ml – 10,000 pg/ml. Sensitivity: < 10 pg/ml.

• Detection method: Absorbance (450 nm) • Species reactivity:mouse • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for mouse Cathepsin D. • Detection Range: 156-10,000 pg/ml. Sensitivity: < 10 pg/ml. • This ELISA kit shows no cross-reactivity with other relevant proteins.

Cathepsin D, CTSD

CTSD (mouse) ELISA Kit

• Cathepsin D Microplate coated with anti-mouse Ab against Cathepsin D, 96 wells • Lyophilized recombinant mouse Cathepsin D standard (10 ng/vial) • Biotinylated anti-mouse Cathepsin D antibody • Avidin-Biotin-Peroxidase Complex (ABC) • Sample diluent buffer • Antibody diluent buffer • ABC diluent buffer • TMB color developing agent (Colorless) • TMB stop solution

-20°C

gel pack

12 months

mouse

Quantitative protein detection, establishing normal range.

• Serum & plasma (heparin, EDTA) • Cell culture supernatants

CTS protease activity also measured by zymograph electrophoresis of Cathepsins. This cathepsin is supplied in 1.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus