Thyroxine (T4) (human) ELISA Kit

T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s human thyroxine (T4) kit is a solid phase competitive ELISA Kit. The samples, assay buffer and T4 enzyme conjugate are added to the wells coated with anti-T4 monoclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for human thyroxine (T4).

Thyroxine, T4

none

• Microplate coated with T4 MAb, 96 wells • T4 Standard: (0.5 ml) (ready to use) • Conjugate Buffer • T4 Enzyme (HRP) Conjugate Concentrate • Wash Concentrate (20X) • TMB Substrate (ready to use) • Stop Solution (ready to use)

2-8°C

gel pack

24 months

human

Quantitative protein detection, establishing normal range.

• Serum or plasma

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED