Testosterone (human) ELISA Kit

Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes; in females ca. 50% of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25% from the ovary and ca. 25% from the adrenal glands. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer. Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter’s syndrome, Testicular feminization, or chidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases. BioVision’s Testosterone EIA kit is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of mouse anti-Testosterone. In the incubation, mouse anti-Testosterone coated wells are incubated with 25 μl of Testosterone standards, controls, samples & 100 μl Testosterone-HRP conjugate reagent at room temperature for 60 min. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 min., resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.

• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for human Testosterone

Testosterone

none

• Microplate coated with Mouse Anti-Testosterone, 96 wells • Standard: (0.5 ml) (ready to use) • Assay Diluent • Enzyme Conjugate (20X) • Wash Buffer (20X) • TMB Substrate (ready to use) • Stop Solution (ready to use)

2-8°C

gel pack

24 months

human

Quantitative protein detection, establishing normal range.

Serum or plasma

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED