Thyroxine (T4) (Mouse/Rat) ELISA Kit

T4 is a useful marker for the diagnosis of hypothyroidism and hyperthyroidism. The level of T4 is decreased in hypothyroid patients and is increased in hyperthyroid patients. BioVision’s mouse/rat thyroxine (T4) kit is a solid phase competitive ELISA Kit. The samples, and T4 enzyme conjugate are added to the wells coated with anti-T4 polyclonal antibody. T4 in the sample competes with a T4 enzyme (HRP) conjugate for binding sites. Unbound T4 and T4 enzyme conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of T4 in the samples. A standard curve is prepared relating color intensity to the concentration of the T4.

• Detection method: Absorbance (450 nm) • Species reactivity: Mouse/Rat • Application: Quantitative protein detection, establishing normal range etc.

• Easy, convenient and time-saving method to assay for mouse/rat thyroxine. • Sensitivity: 0.467 μg/dl.

Thyroxine, T4

none

• Plate coated with T4 Ab • T4 Standard • Assay Diluent • T4 Enzyme Conjugate Conc. • Wash Concentrate (20X) • TMB Substrate • Stop Solution

2-8°C

gel pack

24 months

Mouse/rat

Quantitative protein detection, establishing normal range.

Serum or plasma

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus,Rattus norvegicus

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.