Follicle Stimulating Hormone [FSH] (human) ELISA Kit
Catalog number
K7425-100
Name
Follicle Stimulating Hormone [FSH] (human) ELISA Kit
Size
100 assays
Price
642.00 EUR
Supplier
Details
Kit's description
Follicle Stimulating Hormone (FSH) is a glygoprotein produced by the anterior pituitary gland. In the female, FSH stimulates follicular growth, prepares ovarian follicles for action by LH and enhances the LH induced release of estrogen. FSH levels are elevated after menopause, castration and in premature ovarian failure. Although there are significant exceptions ovarian failure is indicated when random FSH concentrations exceed 40 mIU/ml. In the male, FSH stimulates seminiferous tubule and testicular growth and is involved in the early stages of spermatogenesis. Oligospermic males usually have elevated FSH levels. Tumors of the testes generally depress serum FSH concentrations, but levels of LH are elevated. High levels of FSH in men may be found in primary testicular failure and Klinefelter syndrome. Elevated concentrations are also present in cases of starvation, renal failure, hyperthyroidism, and cirrhosis. BioVision’s human FSH kit is a solid phase sandwich ELISA Kit. The samples, and anti-FSH-HRP conjugate are added to the wells coated with monoclonal antibody to FSH beta subunit. FSH in the sample binds to anti-hFSH MAb on the well and the anti-FSH-HRP second antibody then binds to FSH. Unbound protein and HRP conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of FSH in the samples. A standard curve is prepared relating color intensity to the concentration of the FSH.
Highlights
• Detection method: Absorbance (450 nm) • Species reactivity: human • Application: Quantitative protein detection, establishing normal range etc.
Kit's benefits
• Easy, convenient and time-saving method to measure FSH.
Category
Follicle Stimulating Hormone, FSH
Kit's other name
none
Contents
• Plate coated with FSH MAb • FSH Standard • FSH Enzyme Conjugate • Wash Concentrate (20X) • TMB Substrate • Stop Solution
Storage condition
2-8°C
Shipping condition
gel pack
Maximum time can be stored
24 months
Species reactivity
Human
Applications
Quantitative protein detection, establishing normal range.
Samples tested
Serum
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description
Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.