Lipocalin-2/NGAL (mouse) ELISA Kit

BioVision’s Mouse Lipocalin-2 (LCN2)/NGAL ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Lipocalin-2. This assay employs an antibody specific for mouse Lipocalin-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Lipocalin-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Lipocalin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Lipocalin-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with any of the following cytokines tested: Mouse B7-1, BAFF R, BTC, C5a, CCL6, CD48, CD6, Chemerin, Clusterin, CXCL15, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33, IL-7 R alpha, Kremen-1, Limitin, LOX-1, Marapsin, MBL-2, Meteorin, Nope, NOV, Osteoactivin, OX40, Ligand, P-Cadherin, Periostin, PlGF-2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1, TRAIL, Tryptase epsilon. Sensitivity: 0.02 ng/ml. Detection Range: 0.02 ng/ml – 100 ng/ml. The intra-Assay reproducibility is CV

• Detection method: Absorbance (450 nm) • Species reactivity: Mouse • Application: Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates, and additional reagents allow for use in multiple experiments.

• Easy, convenient and time-saving method to measure mouse Lipocalin-2/NGAL. • Sensitivity: 0.02 ng/ml (range: 0.02-100 ng/ml).

Lipocalin-2 (LCN2)/NGAL ELISA kit

Neutrophil Gelatinase-Associated Lipocalin ELISA Kit, LCN2 ELISA Kit

• Plate coated with anti-mouse Lipocalin-2 • Wash Buffer (20x) (Item B) • Mouse Lipocalin-2 Standard (Item C) • Assay Diluent (Item E) (5x) • Detection Ab Lipocalin-2 (Item F) • HRP-Streptavidin (Item G) (500x) • TMB (Item H) • Stop Solution (Item I)

-20°C

gel pack

12 months

Mouse

Quantitative protein detection, establishing normal range, validation of antibody array results, strip plates, and additional reagents allow for use in multiple experiments.

• Serum & plasma • Cell culture supernatants

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus