TotalExoRNA™ Overall exosome immunocapture and RNA extraction kit (Biological Fluids/Cell Media, 10 reactions)

Overall Exosome Immunocapture and RNA extraction Kit (from human biofluids and cell media)

TotalExoRNA™ Overall exosome immunocapture and RNA extraction kit from biofluids and cell media

High yield of total RNA (including small RNAs)

Exosome

Exosome RNA extraction kit (biofluids/cell media)

Pre-couple latex immunobeads, Bead Washing buffer (5X), Lysis buffer, RNA Washing buffer (to add Ethanol 96%), Elution buffer, Columns, RNase free elution tubes (1.5 ml), Lyophilized Exosome Standard

4°C

gel pack

8 months

Human biological fluids including Plasma, Serum, Urine and Cell culture media.

Direct capture and exosome RNA extraction from human biofluids and cell culture media without initial exosome purification step, • Starting from 0.1 ml of biological sample (plasma) per reaction. Whole plasma and serum can be directly used for capture. Concentrated urine and cell culture supernatant samples are recommended prior capture according to our suggested protocol, Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray)

Human biological fluids including Plasma, Serum, Urine and Cell culture medi

cell, exosome

For cells, cell lines and tissues in culture till half confluency.Differentiation and cell culture media are buffered growth medium solutions or culture medium solutions that can be supplied as a solid or liquid designed to support the growth of microorganisms or cells, or small plants. There are different types of media for growing different types of cells.

Exosomes are 50 nm small lipoprotein vesicles containing lots of microRNAs. The diagnostic value of exosomic miRNAs is due to their role in signal transduction and cell differentiation. Exoslomal total RNA can be analyzed by next gen sequencing to perform a diagnostic micro RNA profile.