PhosphoSeek™ Phosphoprotein Purification Kit

PhosphoSeek™ Phosphoprotein Purification Kit is based on IMAC (immobilized metal affinity chromatography), can isolate the most amount of phosphoprotein from different biological materials, such as animal, plant and microorganism. Isolated phosphoprotein can be Ideal for use in many downstream applications, including mass spectrometry and 2D-PAGE.

• Reactivity: Mammalian, plants, microorganism • Applications: mass spectrometry and 2D:PAGE

• Simple procedure and can purify phosphoprotein within 1 hour. • The kit provides unique formulations of buffers and column to enrich phosphorylated proteins upto 5-10 folds • The purified sample can directly be analyzed by two-dimensional electrophoresis or mass spectrometry.

Phosphoprotein Purification

none

Pre-packed column Lysis buffer System buffer Elution buffer Stringent buffer stock

4°C

gel pack

12 months

see datasheet

Applications- mass spectrometry and 2D-PAGE

Biological materials, such as animal, plant and microorganism

Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. Biovision supplies purification kits and ultra pure reagents.