Fluorimetric Chymotrypsin Activity Assay Kit

Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine). BioVision’s Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin

Fast and convenient, simple procedure: it takes ~ 1-2 hours

Mammalian

Measurement of chymotrypsin activity in tissues / cultured cells.

Chymotrypsin Activity Kit

Fluorescence (Ex/Em= 380/460 nm)

The Fluorimetric Chymotrypsin Activity Assay Kit contains Chymotrypsin Assay Buffer, Chymotrypsin Substrate, Chymotrypsin Activator, Chymotrypsin Inhibitor, Coumarin Standard (1 mM) and Chymotrypsin Positive Control

Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.

Trypsin

Store this kit at -20°C for 12 months

This product is shipped with gel pack

Please contact us to inquire the datasheet and an example certificate of analysis

This assay kit is on the market since May 2017

Biovision supplies other types of Assays as 1.