Colorimetric Glyoxalase I Activity Assay Kit

Glyoxalase I (GloI, lactoylglutathione lyase, EC 4.4.1.5) is a cytosolic enzyme that participates in the Glyoxalase system. The glyoxalase system is a metabolic pathway that detoxifies α-ketoaldehydes such as methylglyoxal to D-lactic acid, via the intermediate S-D-lactoyl-glutathione (SLG). Specifically, GloI catalyzes the formation of SLG from methylglyoxal and reduced Glutathione. It is overexpressed in several human cancers such as colon, breast, prostate and melanoma. GloI also enhances resistance to antitumor agent-induced apoptosis in human leukemia cells. In addition, recent findings have linked GloI to numerous anxiety-like behaviors including psychiatric diseases (depression, schizophrenia and autism) and pain. BioVision’s Glyoxalase I Activity Assay Kit utilizes the ability of an active GloI to catalyze the formation of SLG by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm. Our assay kit is simple, sensitive and can detect as low as 0.05 mU of Glo I activity in biological samples.

Fast and convenient, simple procedure, it takes ~ 40 minutes. The kit contains all necessary reagents for accurate measurement of Glyoxalase I

Mammalian

Measurmente of Glyoxalase I activity in biological samples

Glyoxalase I Activity Kit

Colorimetric (OD 240 nm)

The Colorimetric Glyoxalase I Activity Assay Kit contains GloI Assay Buffer, Glyoxalase I (Lyophilized), GloI Substrate A, GloI Substrate B (Lyophilized) and a 96-well U.V. transparent plate

Cell and tissue lysates,purified enzyme

Glyoxalase II

Store this kit at -20°C for 12 months

This product is shipped with gel pack

Please contact us to inquire the datasheet and an example certificate of analysis

This assay kit is on the market since May 2017

Biovision supplies other types of Assays as 1.

Colorimetric assays or detection use UV absorption or enzymatic color reaction.