Fluorometric PicoProbe Malate Dehydrogenase Activity Assay Kit

Malate Dehydrogenase (MDH) (EC 1.1.1.37) reversibly converts L-Malate into oxaloacetate in the presence of NAD+. In eukaryotic cells, malate dehydrogenase has 2 isoforms, MDH1 and MDH2. MDH1 is cytosolic and participates in the malate-aspartate shuttle, which transports malate into the mitochondria. Malate will then be utilized for the generation of ATP. MDH2, on the other hand, is a mitochondrial enzyme that participates in the citric acid cycle. MDH2 activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma). Biovision’s PicoProbeTM Malate Dehydrogenase Activity Assay Kit (Fluorometric) provides a quick and easy method for monitoring MDH activities in a wide variety of samples. In this assay, MDH converts malate into intermediate products, which will further react with PicoProbeTM forming a fluorophore (Ex/Em=535/587nm). The kit is simple, sensitive and high-throughput adaptable and can detect as low as 40 µU of MDH activity.

Simple, rapid & convenient. The assay can detect Malate Dehydrogenase (MDH) Activity less than 100 µU in various sample types.

Mammalian

• Measurement of malate dehydrogenase activity in various tissues/cells • Analysis of citric acid cycle and malate-asparate shuttle

Malate Dehydrogenase Activity Kit

Fluorescence (535/587 nm)

The Fluorometric PicoProbe Malate Dehydrogenase Activity Assay Kit contains MDH Assay Buffer, MDH Substrate, PicoProbeTM, MDH Developer, NADH Standard and MDH Positive Control

Animal tissues such as liver, heart, muscle, etc. Cell culture: Adherent or suspension cells Mitochondria

Malate Dehydrogenase

Store this kit at -20°C for 12 months

This product is shipped with gel pack

Please contact us to inquire the datasheet and an example certificate of analysis

This assay kit is on the market since May 2017

Biovision supplies other types of Assays as 1.

Picoprobe low capacitance / high speed active probe arms and accessories. These probes arms have a FET amplifier either in or near the tip and are designed for measuring internal node voltages without capacitive loading the circuit