Colorimetric Human Lactate Dehydrogenase A Inhibitor Screening Kit

The human oxidoreductase Lactate Dehydrogenase A (LDHA) (EC:1.1.1.27) is a key enzyme in lactic acid metabolism. It interconverts pyruvate and NADH to lactate and NAD+ respectively. In mammals, LDHA exists as a tetramer and is primarily found in skeletal muscle tissues. LDHA is a common marker to assay for tissue damage and is also known to promote tumor proliferation in breast, lung, and pancreatic cancer patients. In BioVision’s LDHA Inhibitor Screening Kit, LDHA catalyzes the conversion of lactate to an intermediate that interacts with a probe to develop a color that can be measured at OD 450 nm. In the presence of a LDHA specific inhibitor, the reaction is impeded. A LDHA Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is high-throughput adaptable and can be completed in less than 1 hr

Simple and sensitive assay. Fast protocol - can be performed in 30 min. HTP adaptable.

N/A

Screening/characterizing/studying potential inhibitors of Lactate Dehydrogenase A

LDH Inhibitor Screening Kit

Absorbance (OD = 450 nm).

The Colorimetric Human Lactate Dehydrogenase A Inhibitor Screening Kit contains LDHA Assay Buffer, LDHA Substrate Mix, LDHA, LDHA Inhibitor Control and LDHA Dilution Buffer

Small molecules

Metabolism

Store this kit at -20°C for 12 months

This product is shipped with gel pack

Please contact us to inquire the datasheet and an example certificate of analysis

This assay kit is on the market since May 2017

Colorimetric assays or detection use UV absorption or enzymatic color reaction.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.