Mouse Monoclonal Anti-TIF1 gamma Antibody (3H7-C8-B9)

Mouse Monoclonal antibody to detect TIF1γ/TRIM33

Mouse

Monoclonal Antibody (Clone#3H7-C8-B9)

Human

Recombinant human TIF1 gamma / TRIM33 protein fragments expressed in E.coli.

TRIM33 (ECTO, PTC7, RFG7, TF1G, TIF1G, TIF gamma, TIF1gamma)

Epigenetics

51592

Q9UPN9

Colorless liquid

Affinity purified antibody is supplied in liquid form in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol

Affinity purified

Western blot: MCF7 and HeLa nuclear extracts

Western blot; 1:1000

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade)

Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial

Shipped to your lab under gel pack

Manifactured in USA. For Research Use Only! Not to be used in humans

This antibody is available to order since May 2017

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus

anticorps