Mouse Monoclonal Anti-RAD9A Antibody (3A3-A7-F8)

Mouse Monoclonal antibody to detect RAD9A

Mouse

Monoclonal Antibody (Clone#3A3-A7-F8)

Human

Recombinant human RAD9A protein fragments expressed in E.coli

RAD9A (hRAD9, DNA repair exonuclease rad9 homolog A, RAD9A, Cell cycle checkpoint control protein RAD9A)

Epigenetics

5883

Q99638

Colorless liquid

Affinity purified antibody is supplied in liquid form in PBS (pH 7.4) containing with 0.2% sodium azide, 50% glycerol

Affinity purified

Western blot: HeLa, MCF7, 3T3, COS7 and Rat testis cell lysates; Immunoprecipitation: HeLa and 3T3 cells

Western blot; 1:500, Immunoprecipitation

Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.

Before use gently mix the antibody solution. Store this antibody at -20 °C for 12 months. Refer to the label on vial

Shipped to your lab under gel pack

Manifactured in USA. For Research Use Only! Not to be used in humans

This antibody is available to order since May 2017

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Mus musculus

anticorps